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pires2 dsred express 2 vector  (TaKaRa)


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    Structured Review

    TaKaRa pires2 dsred express 2 vector
    Pires2 Dsred Express 2 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 225 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pires2+dsred+express/pIRES2+DsRed-Express2+Vector/us12496274-2463-13-17
    Average 94 stars, based on 225 article reviews
    pires2 dsred express 2 vector - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Purification:

    Article Title: ATP synthase subunit e is a shrimp growth-associated breeding marker.
    Article Snippet: .. The purified DNA was quantified using NanoDrop One spectrophotometer (Thermo Fisher, USA), then ligated into the pIRES2-DsRed-Express with BamH1 and Xho1 restriction enzymes (TaKaRa, Japan), and transformed into E. coli DH5α (Weidi, Shanghai, China). .. The recombinant plasmids were confirmed by PCR and sequenced by Beijing Genomics Institute (BGI, Beijing, China).

    Spectrophotometry:

    Article Title: ATP synthase subunit e is a shrimp growth-associated breeding marker.
    Article Snippet: .. The purified DNA was quantified using NanoDrop One spectrophotometer (Thermo Fisher, USA), then ligated into the pIRES2-DsRed-Express with BamH1 and Xho1 restriction enzymes (TaKaRa, Japan), and transformed into E. coli DH5α (Weidi, Shanghai, China). .. The recombinant plasmids were confirmed by PCR and sequenced by Beijing Genomics Institute (BGI, Beijing, China).

    Transformation Assay:

    Article Title: ATP synthase subunit e is a shrimp growth-associated breeding marker.
    Article Snippet: .. The purified DNA was quantified using NanoDrop One spectrophotometer (Thermo Fisher, USA), then ligated into the pIRES2-DsRed-Express with BamH1 and Xho1 restriction enzymes (TaKaRa, Japan), and transformed into E. coli DH5α (Weidi, Shanghai, China). .. The recombinant plasmids were confirmed by PCR and sequenced by Beijing Genomics Institute (BGI, Beijing, China).

    Polymerase Chain Reaction:

    Article Title: A Genome-Wide Analysis of FRT -Like Sequences in the Human Genome
    Article Snippet: The amplified EGFP gene was cloned into pcDNA5/FRT (Invitrogen) under the control of the CMV promoter between NheI and HindIII to obtain pcDNA5-EGFP. .. Then, the DsRed gene from pIRES2-DsRed-Express (Clontech) was PCR-amplified and cloned into pcDNA5-EGFP between BamHI and XhoI to obtain pEGFP-del. .. The mammalian expression vectors for Flp variants were constructed by cloning the genes for the variants into pOG44 (Invitrogen) in place of the FLP -F70L variant gene.

    Clone Assay:

    Article Title: A Genome-Wide Analysis of FRT -Like Sequences in the Human Genome
    Article Snippet: The amplified EGFP gene was cloned into pcDNA5/FRT (Invitrogen) under the control of the CMV promoter between NheI and HindIII to obtain pcDNA5-EGFP. .. Then, the DsRed gene from pIRES2-DsRed-Express (Clontech) was PCR-amplified and cloned into pcDNA5-EGFP between BamHI and XhoI to obtain pEGFP-del. .. The mammalian expression vectors for Flp variants were constructed by cloning the genes for the variants into pOG44 (Invitrogen) in place of the FLP -F70L variant gene.

    Expressing:

    Article Title: Cellular Pathophysiology of Mutant Voltage-Dependent Ca2+ Channel CACNA1H in Primary Aldosteronism.
    Article Snippet: .. CACNA1H expression vectors Plasmid (pcDNA3 [ampR]) containing the human wildtype CACNA1H (Cav3.2a) cDNA sequence (GenBank accession number AF051946), a gift from Dr. Edward Perez-Reyes (University of Virginia, Charlottesville, VA; Addgene plasmid ID45809 (55)), was used as template for subcloning the coding sequence of CACNA1H into pIRES2-DsRed-Express (Clontech Laboratories, Mountain View, CA). ..

    Article Title: Tools for Co-expressing Multiple Proteins in Mammalian Cells
    Article Snippet: .. 2.1 Construction of expression vectors pFM1.2 ( 15 ) ( see Note 1 ). pIRES2-DsRed-Express (Clontech, cat. no. 632463) ( see Note 2 ). pASK84 ( 16 ), pBluescript (Stratagene, cat. nos. .. 212205-8), and Fv fragments cloned in pGEM-T easy (Promega, cat.no A1360) ( see Note 3 ). pPURO ( see Note 4 ).

    Plasmid Preparation:

    Article Title: Cellular Pathophysiology of Mutant Voltage-Dependent Ca2+ Channel CACNA1H in Primary Aldosteronism.
    Article Snippet: .. CACNA1H expression vectors Plasmid (pcDNA3 [ampR]) containing the human wildtype CACNA1H (Cav3.2a) cDNA sequence (GenBank accession number AF051946), a gift from Dr. Edward Perez-Reyes (University of Virginia, Charlottesville, VA; Addgene plasmid ID45809 (55)), was used as template for subcloning the coding sequence of CACNA1H into pIRES2-DsRed-Express (Clontech Laboratories, Mountain View, CA). ..

    Article Title: SpiAMT1d: molecular characterization, localization, and potential role in coral calcification of an ammonium transporter in Stylophora pistillata
    Article Snippet: Members of the ammonium transporter family occur in all domains of life.. However, they have been characterized, at the molecular level, mainly in bacteria and plants, whereas in animals their molecular characterization is limited to a few species.. In marine invertebrates, recent studies have reported a multitude of physiological functions in which ammonium could take part.

    Article Title: Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals.
    Article Snippet: .. To generate the sleeping beauty donor vector with an N-terminal 3xFLAG tag and a fluorescent readout of doxycycline induction, we first used primers with overhangs harboring SfiI sites to amplify the IRES-DsRedExpress from pIRES2-DsRed-Express (Clontech)(Supplementary Data 5). ..

    Sequencing:

    Article Title: Cellular Pathophysiology of Mutant Voltage-Dependent Ca2+ Channel CACNA1H in Primary Aldosteronism.
    Article Snippet: .. CACNA1H expression vectors Plasmid (pcDNA3 [ampR]) containing the human wildtype CACNA1H (Cav3.2a) cDNA sequence (GenBank accession number AF051946), a gift from Dr. Edward Perez-Reyes (University of Virginia, Charlottesville, VA; Addgene plasmid ID45809 (55)), was used as template for subcloning the coding sequence of CACNA1H into pIRES2-DsRed-Express (Clontech Laboratories, Mountain View, CA). ..

    Article Title: SpiAMT1d: molecular characterization, localization, and potential role in coral calcification of an ammonium transporter in Stylophora pistillata
    Article Snippet: Members of the ammonium transporter family occur in all domains of life.. However, they have been characterized, at the molecular level, mainly in bacteria and plants, whereas in animals their molecular characterization is limited to a few species.. In marine invertebrates, recent studies have reported a multitude of physiological functions in which ammonium could take part.

    Subcloning:

    Article Title: Cellular Pathophysiology of Mutant Voltage-Dependent Ca2+ Channel CACNA1H in Primary Aldosteronism.
    Article Snippet: .. CACNA1H expression vectors Plasmid (pcDNA3 [ampR]) containing the human wildtype CACNA1H (Cav3.2a) cDNA sequence (GenBank accession number AF051946), a gift from Dr. Edward Perez-Reyes (University of Virginia, Charlottesville, VA; Addgene plasmid ID45809 (55)), was used as template for subcloning the coding sequence of CACNA1H into pIRES2-DsRed-Express (Clontech Laboratories, Mountain View, CA). ..

    Construct:

    Article Title: Feedback control of protein expression in mammalian cells by tunable synthetic translational inhibition
    Article Snippet: .. Plasmids containing IRES-EGFP (or ECFP) were constructed as follows: first, pIRES2-EGFP (pIRES2-ECFP) was constructed by replacing the DsRed-Express of pIRES2-DsRed-Express (Clontech, Mountain View, CA) with EGFP (or ECFP). ..



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    Image Search Results


    Journal: iScience

    Article Title: Photoswitchable TRPC6 channel activators evoke distinct channel kinetics reflecting different gating behaviors

    doi: 10.1016/j.isci.2024.111008

    Figure Lengend Snippet:

    Article Snippet: The cDNA was in pIRES2-EGFP expression vector (Clontech, Palo Alto, CA).

    Techniques: Recombinant, Expressing, Plasmid Preparation, Software, Inverted Microscopy, Microscopy, Control, Spectrophotometry